Interphase is the preparatory phase between two successive M phases. Though called the 'resting phase', the cell is highly metabolically active. It comprises three sub-phases:
G1 phase (Gap 1 / First Growth phase):
- Corresponds to the interval between the end of the previous M phase and the start of DNA replication.
- The cell grows in size; metabolic activity is high.
- Organelle duplication (other than centrioles) occurs.
- DNA is NOT yet replicated.
- Cells that exit the cycle permanently enter the G0 (quiescent) stage from here.
S phase (Synthesis phase):
- DNA replication (synthesis) takes place; the amount of DNA per cell doubles from 2C to 4C.
- Chromosome number remains the same (2n — no new chromosomes are added; each chromosome now consists of two identical sister chromatids).
- In animal cells, centrioles also duplicate during this phase.
G2 phase (Gap 2 / Second Growth phase):
- DNA replication is complete; the cell checks for errors.
- Proteins required for mitosis (e.g., tubulin for spindle fibres) are synthesised.
- Cell growth continues in preparation for division.